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首頁 ∷ 試劑 ∷ RD ∷ Human alpha-Smooth Muscle Actin PE-conjugated Antibody

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Human alpha-Smooth Muscle Actin PE-conjugated Antibody

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商品描述

Human alpha-Smooth Muscle Actin PE-conjugated Antibody,IC1420P,R&D:

Human alpha-Smooth Muscle Actin PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha -Smooth Muscle Actin.

Source

Monoclonal Mouse IgG_2A Clone # 1A4

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 μL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of alpha ?Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry.

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Detection of alpha ?Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human alpha ?Smooth Muscle Actin PE?conjugated Monoclonal Antibody (Catalog # IC1420P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha ?Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References

Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
Oishi, K. et al. (2002) J. Physiol. 540:139.

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Entrez Gene IDs

59 (Human); 11475 (Mouse); 81633 (Rat)

Alternate Names

AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

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