Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross?reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Sodium Phosphate and Proclin 300.
Label
HRP (Horseradish Peroxidase)
Applications
Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse IgG Primary Antibody byWestern Blot Westernblot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody
(Catalog #MAB1799)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody
(Catalog # HAF007). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Reconstitution Calculator
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
6 months from date of receipt, 2 to 8 °C as supplied.
