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Thromboxane B2 Parameter Assay Kit ,KGE011,R&D：

Thromboxane B2 Parameter Assay Kit Summary

Format

96-well strip plate

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)

Sensitivity

0.31 ng/mL

Assay Range

0.3 - 20 ng/mL (Cell Culture Supernates, Serum, Plasma, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Measures TXB₂ levels in various samples

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Parameter TXB₂ Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure TXB₂ in cell culture supernates, serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	3.87	6.95	13.2	3.86	7.05	14.4
Standard Deviation	0.23	0.38	0.51	0.34	0.45	0.74
CV%	5.9	5.5	3.9	8.9	6.4	5.1

Recovery

The recovery of TXB₂ spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	88-110
EDTA Plasma (n=4)	94	86-105
Heparin Plasma (n=4)	97	87-107
Serum (n=4)	98	86-111
Urine (n=4)	98	84-111

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TXB₂ were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Data Examples

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Multi-species Thromboxane B2 ELISA Standard Curve