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Human GM-CSF
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商品描述
Human GM-CSF ，130-093-865，MiltenyiBiotec:
overview：
Details:
overview：
GM-CSF stands for human granulocyte-macrophage colony–stimulating factor. Human GM-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Applications
Human GM-CSF can be used for a variety of applications including:
- Cultivation of hematopoietic progenitor cells from human bone marrow in semi-solid medium.
- In vitro generation of Mo-DCs together with Human IL-4.
- In vitro differentiation of CD34+ cells towards eosinophils.
- Migration assays for eosinophils.
Alternative names
CSF2
Details:
Background information
GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF is a potent chemoattractant for neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request ([email protected]).
Biological activity
-
Proliferation of TF-1 cells (NIBSC 88/646)
-
premium grade: ≥ 5×
10
6
IU/mg
(typical activity: ≥ 1.2×
10
7
IU/mg
)
-
research grade: ≥ 2×
10
6
IU/mg
GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF is a potent chemoattractant for neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request ([email protected]).
Proliferation of TF-1 cells (NIBSC 88/646)
premium grade: ≥ 5×
10
6
IU/mg
(typical activity: ≥ 1.2×
10
7
IU/mg
research grade: ≥ 2×
10
6
IU/mg
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100T
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1
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1kit
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autoMACS
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1500ml
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autoMACS
Rinsing Solution
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6×1.45L
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Columns with plungers
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1kit
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1kit
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24
bags
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200-074-401
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