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PepTivator CMV pp65, human

PepTivator CMV pp65, human

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商品描述
PepTivator CMV pp65, human，130-093-438，MiltenyiBiotec：
overview：
PepTivator
?
CMV pp65 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the pp65 protein of human cytomegalovirus (UniProt ID: P06725).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen-specific CD4
+
and CD8
+
T cells, as peptides of 15–aa length with 11–aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
Quantitative, phenotypical, or functional analysis of pp65-specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.
PepTivator CMV pp65-HT is a peptide pool lyophilized in the wells of a 96-well plate, allowing stimulation and analysis of cells directly on the plate.
The convenient 96-well format, composed of 12 individually removable strips of 8 wells each allows for easy and flexible experimental set-up, different antigens can be combined by assembling different strips. A control can also be added easily by using strips from the Control plate (12x8).


Details:

Background information

CMV pp65 (65 kDa lower matrix phosphoprotein), also known as glycoprotein 64 or UL83, is a virion tegument protein and the main component of the enveloped subviral particle. CMV pp65 is an immunodominant target of CD4
+
and CD8
+
T cell responses to CMV. CMV pp65-specific T cells predominantly produce inflammatory cytokines, such as IFN-γ, IL-2, and TNF-α.

Downstream applications

The
in vitro
stimulation of pp65-specific CD4
+
and CD8
+
T cells with PepTivator CMV pp65 causes the secretion of effector cytokines and the upregulation of activation markers, which then allow the detection and isolation of pp65-specific T cells
1-6
:
  • Detection and analysis of CMV pp65-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS? Cytokine Secretion Assays, intracellular staining, or other technologies.
  • Isolation of viable CMV pp65-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay - Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of CMV pp65-specific CD4+ and CD8+ effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells for research on dendritic cell vaccination.
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規(guī)格型號(hào)
M/MiltenyiBiotec
130-042-201
MS Columns
25個(gè)
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130-042-303
MACS MultiStand
1 piece
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130-042-401
LS Columns
25 columns
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130-045-801
CD45 MicroBeads, human
1kit
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130-046-703
CD34 microbead kit
10*10
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130-048-401
Anti-Mouse IgG MicroBeads
2ml
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130-048-801
Anti-PE MicroBeads
1kit
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130-050-201
CD14 MicroBeads, human
1kit
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130-050-401
CD56 MircroBeads,human
1ml
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130-050-501
CD235a (Glycophorin A) MicroBeads, human
1kit
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130-090-101
Dead Cell Removal Kit
1kit
M/MiltenyiBiotec
130-090-433
IFN-γ Secretion Assay – Detection Kits, human
100T
M/MiltenyiBiotec
130-090-976
QuadroMACS Separator
1 piece
M/MiltenyiBiotec
130-091-051
QuadroMACS Starting Kit (LS)
1kit
M/MiltenyiBiotec
130-091-052
MACS 15 mL Tube Rack
1PK
M/MiltenyiBiotec
130-091-221
autoMACS Rinsing Solution
1500ml
M/MiltenyiBiotec
130-091-222
autoMACS Rinsing Solution
6×1.45L
M/MiltenyiBiotec
130-091-376
MACS BSA Stock Solution
6x75ml
M/MiltenyiBiotec
130-093-438
PepTivator CMV pp65, human
1EA
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130-093-865
human GM-CSF (premium)
50ug
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human IL3
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human IL6 (premium)
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human TPO
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CD14-PerCP, human
100T
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human IL7 (premium)
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Tumor Dissociation Kit, human
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human FLT3 (premium)
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human SCF (premium)
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human IL-11
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CD45-PE, rat
200T/支
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130-110-905
μ Columns with plungers
1kit
M/MiltenyiBiotec
130-111-573
Exosome Starting Kit CD9, human
1kit
M/MiltenyiBiotec
200-074-400
Freezing Bags
24 bags
M/MiltenyiBiotec
200-074-401
Freezing Bags
24 bags