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豬胰島素(INS)ELISA Kit CSB-E06828p

豬胰島素(INS)ELISA Kit CSB-E06828p

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商品描述
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    0 1.800 1.864 1.832    
    2 1.569 1.581 1.575    
    8 1.304 1.327 1.316    
    32 0.812 0.805 0.809    
    100 0.389 0.393 0.391    
    400 0.180 0.183 0.182    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 84-105  
    1:2 Average % 93  
    Range % 89-99  
    1:4 Average % 95  
    Range % 89-101  
    1:8 Average % 95  
    Range % 83-103  
  • 回收率:
    The recovery of pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-101  
    EDTA plasma (n=4) 98 92-104  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領(lǐng)域:
    Metabolism