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人胎盤(pán)堿性磷酸酶(PLAP)ELISA Kit CSB-E09160h

人胎盤(pán)堿性磷酸酶(PLAP)ELISA Kit CSB-E09160h

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商品描述

  • 產(chǎn)品類型:
    ELISA Kit
  • 產(chǎn)品名稱:

    人胎盤(pán)堿性磷酸酶(PLAP)ELISA Kit

  • 英文名稱:

    Human Placental alkaline phosphatase,PLAP ELISA Kit

  • 貨號(hào):
    CSB-E09160h
  • 別名:
    ALP, FLJ61142, PALP, PLAP, alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase
  • 規(guī)格:
    96T
  • 中文價(jià)格:
    3200
  • 種屬:
    Homo sapiens (Human)
  • 待測(cè)物名稱:
    alkaline phosphatase, placental (Regan isozyme)
  • 縮寫(xiě):
    ALPP
  • 檢測(cè)范圍:
    84 pg/mL-2000 pg/mL
  • 靈敏度:
    34 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.012 1.983 1.998 1.952  
    1000 1.338 1.341 1.340 1.294  
    500 0.775 0.828 0.802 0.756  
    167 0.357 0.349 0.353 0.307  
    84 0.224 0.235 0.230 0.184  
    0 0.047 0.045 0.046  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<10%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-98  
    1:2 Average % 96  
    Range % 88-100  
    1:4 Average % 95  
    Range % 89-100  
    1:8 Average % 97  
    Range % 91-101
  • 回收率:
    The recovery of human PLAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-104  
    EDTA plasma (n=4) 95 91-101  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領(lǐng)域:
    Tags & Cell Markers