This product should be stored undiluted.
Storage in frost free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Purified IgG was prepared from serum by ion exchange chromatography.
Clonality
Polyclonal
Isotype
IgG
Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody (ab11713)This image is courtesy of an anonymous abreview.
ab11713 at a 1/1000 dilution staining Von Willebrand Factor in mouse blood vessels by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 1 hour at 19°C. Secondary used at 1/200 polyclonal Donkey anti-sheep conjugated to Alexa Fluor 647 (pink). Counterstain (blue) DAPI.
Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody (ab11713)Image courtesy of an anonymous Abreview.
ab11713 staining Von Willebrand Factor in porcine heart tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in acetone, permeabilized using 0.3% Triton/PBS, blocked with 10% serum for 30 minutes at 22°C and then incubated with ab11713 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a FITC conjugated donkey anti-sheep polyclonal used at a 1/1000 dilution.
