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All lanes : Anti-Claudin 3 antibody (ab116165) at 1 μg/ml

Lane 1 : Human ovary tissue lysate - total protein (ab30222)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : Human ovary tissue lysate - total protein (ab30222) with Immunizing peptide at 1 μg/ml
Lane 7 : Human colon tissue lysate - total protein (ab30051) with Immunizing peptide at 1 μg/ml
Lane 8 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 μg/ml
Lane 9 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 μg/ml
Lane 10 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 μg/ml

Lysates/proteins at 10 μg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 23 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.


Exposure time: 16 minutes

IHC image of Claudin 3 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116165, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product name

Anti-Claudin 3 antibody
See all Claudin 3 primary antibodies

Description

Rabbit polyclonal to Claudin 3

Host species

Rabbit

Tested applications

Suitable for: WB,IHC-P

Species reactivity

Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Claudin 3.

Read Abcam's proprietary immunogen policy

Positive control

• This antibody gave a positive signal in both Human Ovary and Human Colon tissue lysates as well as the following whole cell lysates: Caco2; SW480; LoVo. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal prostate.

Form

Liquid

Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG