產(chǎn)品簡介

RFect小核酸轉(zhuǎn)染試劑是知名科學(xué)家崔坤元博士領(lǐng)導(dǎo)我公司研發(fā)團(tuán)隊(duì)在美國西雅圖實(shí)驗(yàn)室研發(fā)成功的一種新型的小核酸轉(zhuǎn)染試劑。RFect可用來轉(zhuǎn)染siRNA、miRNA mimics、miRNA inhibitor 等200bp以內(nèi)的小分子RNA，轉(zhuǎn)染細(xì)胞包括絕大多數(shù)貼壁生長的細(xì)胞，如一般細(xì)胞株、腫瘤細(xì)胞株等。目前，無論國外還是國內(nèi)，轉(zhuǎn)染試劑的主要成分均為脂質(zhì)體或聚乙烯亞胺(Polyethylenimine, PEI)，這兩種成分都具有很大的細(xì)胞毒性，并且轉(zhuǎn)染效果不好。RFect采用新型的動(dòng)物源性的納米材料，毒性很低并且擁有非常高的轉(zhuǎn)染性能。與其它品牌的小核酸轉(zhuǎn)染試劑相比，RFect的細(xì)胞轉(zhuǎn)染陽性率一般在90%以上，Lamin A/C 基因抑制效率在95%以上，而其它品牌轉(zhuǎn)染試劑的細(xì)胞轉(zhuǎn)染陽性率一般不超過70%，Lamin A/C 基因抑制效率不超過75%。RFect細(xì)胞毒性很低，轉(zhuǎn)染細(xì)胞死亡率不到10%，而其它品牌試劑的轉(zhuǎn)染細(xì)胞死亡率一般在30%以上。血清對(duì)轉(zhuǎn)染效果沒有影響，不必刻意添加或更換培養(yǎng)液。本產(chǎn)品適用于細(xì)胞株轉(zhuǎn)染，原代細(xì)胞轉(zhuǎn)染，請(qǐng)選擇RFectPM小核酸轉(zhuǎn)染試劑。

產(chǎn)品特點(diǎn)

·細(xì)胞轉(zhuǎn)染性能：細(xì)胞轉(zhuǎn)染陽性率高達(dá)90%以上，基因敲除效果明顯，A549細(xì)胞Lamin A/C 基因敲除效率在95%以上；

· 極低的細(xì)胞毒性：轉(zhuǎn)染細(xì)胞死亡率不到10%，大大降低了因細(xì)胞毒性對(duì)實(shí)驗(yàn)結(jié)果的影響；

· 轉(zhuǎn)染細(xì)胞范圍廣，絕大多數(shù)貼壁細(xì)胞株都能獲得比較理想的轉(zhuǎn)染結(jié)果。

Fig. 1. RFect sRNA transfection reagent offers the highest transfection efficiency.  HeLa cells were transfected with Alexa 546-dye labeled siRNA at final concentration of 10nM using RFect sRNA transfection reagent. After 24 hrs, the cells were stained with Hoechst 33342 (nuclear staining dye) and then were viewed by fluorescence microscopy. A The fluorescence images show the fluorescence of labeled siRNA.B.The fluorescence images show the fluorescence of labeled siRNA (in red) overlaid with fluorescence from Hoechst 33342 nuclear staining (in blue).

Fig..2. RFect siRNA transfection reagent offers the highest level of gene knockdown on a variety of cell types. Different cell lines were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFect  In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

Fig..3.RFect siRNA transfection reagent offers high level of gene knockdown with low toxicity. A549 cell lines were transfected with Lamin A/C siRNA (final concentration: 10nM) using RFect siRNA transfection reagent. RFect concentrations added to the cells  exceeding transfection concentration of 20 times, the cells still do not produce significant toxicity, which makes these product suitable to optimization. Lamin A/C knockdown was measured by qRT-PCR.

Fig.4.RFect siRNA transfection reagent offers consistent high level of gene knockdown despite differences in cell density.A549 cells at different cell density were transfected with Lamin siRNA (final concentration: 10nM) using RFect.

適用細(xì)胞

293T（人腎上皮細(xì)胞），A549（人肺癌細(xì)胞），HEK 293（人胚腎細(xì)胞），HeLa（人宮頸癌細(xì)胞），MCF-7（人乳腺癌細(xì)胞），HepG2（人肝腫瘤細(xì)胞），HT-1080(人纖維肉瘤），HLF-a（人肺細(xì)胞），Huh-7（人肝癌細(xì)胞），KB（人口腔表皮樣癌細(xì)胞），PC3（人前列腺癌細(xì)胞），BRL-3A（大鼠正常肝細(xì)胞），DU 145（人前列腺癌細(xì)胞），Hepa 1-6（小鼠肝癌細(xì)胞），OVCAR3（人卵巢癌細(xì)胞），NCaP-FGC（人前列腺癌細(xì)胞），C2C12（成肌細(xì)胞），MDCK（犬腎細(xì)胞），CHO-K1，islk.219細(xì)胞（人肺癌細(xì)胞），HT-29（人結(jié)腸癌細(xì)胞），NCI-H1688（人典型小細(xì)胞肺腫瘤/癌細(xì)胞），RKO結(jié)腸癌細(xì)胞，Vero（非洲綠猴腎細(xì)胞），COS-1（非洲綠猴腎成纖維細(xì)胞），COS7-L（非洲綠猴腎成纖維細(xì)胞），COS-7（非洲綠猴腎成纖維細(xì)胞），SK-BR3（人乳腺癌細(xì)胞），Hepa 1cLc7（鼠肝癌細(xì)胞），Daoy（人髓母細(xì)胞瘤細(xì)胞），DBTRG-05MG（人腦膠質(zhì)母細(xì)胞瘤細(xì)胞），KLN 205（鼠肺磷癌細(xì)胞），Neuro-2a（小鼠神經(jīng)瘤母細(xì)胞），DI-TNC1（大鼠腦間質(zhì)細(xì)胞）等。

相關(guān)文獻(xiàn)

1.  Basic Research in Cardiology(2021). HIMF deletion ameliorates acute myocardial ischemic injury by promoting macrophage transformation to reparative subtype. (IF:11.981) DOI: 10.1007/s00395-021-00867-7

2.   NUCLEIC ACIDS RESEARCH(2021). XPD localizes in mitochondria and protects the mitochondrial genome from oxidative DNA damage. (IF:11.24 ) DOI: 10.1093/nar/gkv472

3.  MYO1F regulates antifungal immunity by regulating acetylation of microtubules. (IF:11.205) DOI: 10.1073/pnas.2100230118

4.  Cellular & Molecular Immunology(2021). The miR-628-5p Facilitates Enterovirus 71 Infection by Suppressing TRAF3-Mediated p65 and IRF3 Signaling. (IF:8.484) DOI: 10.1038/s41423-020-0453-4

5.  Levobupivacaine Induces Ferroptosis by miR-489-3p/SLC7A11 Signaling in Gastric Cancer. (IF:5.81) DOI: 10.3389/fphar.2021.681338

6.  Proteome Analysis in PAM Cells Reveals That African Swine Fever Virus Can Regulate the Level of Intracellular Polyamines to Facilitate Its Own Replication through ARG1. (IF:5.046) DOI: 10.3390/v13071236

7.  AGING-US(2021). Extracellular vesicles from anoxia preconditioned mesenchymal stem cells alleviate myocardial ischemia/reperfusion injury. (IF:4.909 ) DOI: 10.18632/aging.202611

8.  International Journal of Biological Sciences(2021).DGCR5 Promotes Gallbladder Cancer by Sponging MiR-3619-5p via MEK/ERK1/2 and JNK/p38 MAPK Pathways. (IF:4.773) DOI: 10.7150/jca.46351

9.  International Journal of Biological Sciences(2021).Rac GTPase activatingprotein 1 promotes gallbladder cancer via binding DNA ligase 3 to reduce apoptosis. (IF:4.773) DOI: 10.7150/ijbs.58857

10.  International Journal of Biological Sciences(2021).ESRRA promotes gastric cancer development by regulating the CDC25C/CDK1/CyclinB1 pathway via DSN1. (IF:4.773)  DOI:10.7150/ijbs.57623

11.  International Journal of Biological Sciences(2021).KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. (IF:4.773 ) DOI: 10.7150/ijbs.54074

12.  International Journal of Biological Sciences(2021). DGCR5 is activated by PAX5 and promotes pancreatic cancer via targeting miR-3163/TOP2A and activating Wnt/β-catenin pathway.(IF:4.773 ) DOI: 10.7150/ijbs.55636

13.  Journal of Cellular and Molecular Medicine(2021). Down‐regulation of RCC1 sensitizes immunotherapy by up‐regulating PD‐L1 via p27kip1/CDK4 axis in non‐small cell lung cancer. (IF:4.438) DOI: 10.1111/jcmm.16383

14.  scientific reports(2021). Clinicalsignificance of Annexin A2 e­xpression in oral squamous cell carcinoma and its influence on cell proliferation, migration and invasion. (IF:4.149) DOI: 10.1038/s41598-021-84675-y

15.  Journal of Cancer(2021). LncRNALOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1. (IF:3.565) DOI: 10.7150/jca.51136

16.  Environmental Geochemistry and Health(2021).Inorganic arsenic influences cell apoptosis by regulating the e­xpression of MEG3 gene. (IF:3.472 ) DOI: 10.1007/s10653-020-00740-x

17.  Bta-miR-223 Targeting CBLB Contributes to Resistance to Staphylococcus aureus Mastitis Through the PI3K/AKT/NF-κB Pathway. (IF:3.412) DOI: 10.3389/fvets.2020.00529

18.  Molecular Human Reproduction(2021).Excess fibronectin 1 participates in pathogenesis of pre-eclampsia by promoting apoptosis and autophagy in vascular endothelial cells. (IF:3.469) DOI: 10.1093/molehr/gaab030

19.  American Journal of Translational Research(2021). Irg1-itaconateaxis protectsagainst acute kidney injury via activation of Nrf2. (IF:3.375 )

20.  ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH(2021).Arsenic exposure increased e­xpression of HOTAIR and LincRNA-p21 in vivo and vitro. (IF:3.33) DOI: 10.1007/s11356-020-10487-8

21.  Environmental Toxicology and Pharmacology(2021).Long non-coding RNA DICER1-AS1-low e­xpression in arsenic-treated A549 cells inhibits cell proliferation by regulating the cell cycle pathway. (IF:3.292 ) DOI: 10.1016/j.etap.2021.103617

22.  ENVIRONMENTAL TOXICOLOGY(2021).Inorganic arsenic‐mediated upregulation of AS3MT promotes proliferation of nonsmall cell lung cancer cells by regulating cell cycle genes. (IF:3.118 ) DOI: 10.1002/tox.23026

23.  Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells. (IF:2.59) DOI: 10.1590/1414-431x2021e11156

24.  FEBS Open Bio(2021).SREBP1/FASN/cholesterol axis facilitates radioresistance in colorectal cancer.  (IF:2.231 ) DOI: 10.1002/2211-5463.13137

25.  Clinical And Translational Medicine(2020).Long noncoding RNA lncGALM increases risk of liver metastasis in gallbladder cancer through facilitating N‐cadherin and IL‐1β‐dependent liver arrest and tumor?extravasation. (IF:7.919 ) DOI: 10.1002/ctm2.201

26.  Clinical And Translational Medicine(2020).Mir‐27a‐3p attenuates bronchiolitis obliterans in vivo via the regulation of dendritic cells' maturation and the suppression of myofibroblasts' differentiation. (IF:7.919 ) DOI: 10.1002/ctm2.140

27.  Journal of Experimental & Clinical Cancer Research(2020).Loss of miR-192-5p initiates a hyperglycolysis and stemness positive feedback in hepatocellular carcinoma. (IF:7.068) DOI: 10.1186/s13046-020-01785-7

28.  American Journal of Cancer Research(2020).ERRα promotes pancreatic cancer progression by enhancing the transcription of PAI1 and activating the MEK/ERK pathway.  (IF:5.884 )

29.   Frontiers in Cell and Developmental Biology(2020).CRLF1 is a key regulator in the ligamentum flavum hypertrophy. (IF:5.8 ) DOI: 10.3389/fcell.2020.00858

30.   Cancer Science(2020). Anthrax toxin receptor 1/tumor endothelial marker 8 promotes gastric cancer progression through activation of the PI3K/AKT/mTOR signaling pathway. (IF:4.985) DOI: 10.1111/cas.14326

31.  Journal of Cellular and Molecular Medicine(2020). Down‐regulation of RCC1 sensitizes immunotherapy by up‐regulating PD‐L1 via p27kip1/CDK4 axis in non‐small cell lung cancer. (IF:4.438 ) DOI: 10.1111/jcmm.16383

32.  Frontiers in Pharmacology(2020). Long Noncoding RNA LINC-PINT Suppresses Cell Proliferation, Invasion, and EMT by Blocking Wnt/β-Catenin Signaling in Glioblastoma. (IF:4.225) DOI: 10.3389/fphar.2020.586653

33.  Fish Shellfish Immunology(2020).Chlorpyrifos induces apoptosis and autophagy in common carp lymphocytes by influencing the TCR γ-dependent PI3K/AKT/JNK pathway. (IF:4.149) DOI: 10.1016/j.fsi.2020.02.039

34.  Journal of Biological Chemistry(2020).Estrogen-induced FOS-like 1 regulates matrix metalloproteinase e­xpression and the motility of human endometrial and decidual stromal cells. (IF:3.311 ) DOI: 10.1074/jbc.RA119.010701

35.  Journal of Fish Diseases(2020).Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ.  (IF:2.323) DOI: 10.1111/jfd.13138

36.  Molecular Medicine Reports(2020). Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3_x005f_x001e_E1 cells via an autocrine mechanism. (IF:2.085) DOI: 10.3892/mmr.2020.11506

37.  Chinese Medical Journal(2020).Topoisomerase IIalpha promotes gallbladder cancer proliferation and metastasis through activating phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway.  (IF:1.585) DOI: 10.1097/CM9.0000000000001075

38.  Autophagy(2019).USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy. (IF:11.06 )  DOI: 10.1080/15548627.2019.1656957

39.  Oxidative Medicine and Cellular Longevity(2019).Aloin preconditioning attenuates hepatic ischemia/reperfusion injury via inhibiting TLR4/MyD88/NF-κB signal pathway in vivo and in vitro.  (IF:5.163 ) DOI: 10.1155/2019/3765898

40.  Oxidative Medicine and Cellular Longevity.(2019)Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite.(IF:5.076)DOI: 10.1155/2019/5769752

41.  Aging(2019).Circular RNA CircCCNB1 sponges micro RNA-449a to inhibit cellular senescence by targeting CCNE2. (IF:4.909 ) DOI: 10.18632/aging.102449

42.  International Journal of Biological Sciences(2019).Glycolytic enzyme PKM2mediates autophagic activation to promote cell survival in NPM1-mutated leukemia. (IF:4.773) DOI: 10.7150/ijbs.30290

43.  Cancer Science(2019).NOP2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL6 in gallbladder carcinoma. (IF:4.751 ) DOI: 10.1111/cas.14190

44.  International Journal of Molecular Sciences(2019).Comparison of LncRNA e­xpression profiles during myogenic differentiation and adipogenic transdifferentiation of myoblasts. (IF:4.602) DOI: 10.3390/ijms20153725

45.  Biomed Pharmacother(2019).LncRNA MNX1-AS1 promotes progression of esophageal squamous cell carcinoma by regulating miR-34a/SIRT1 axis. (IF:3.743) DOI: 10.1016/j.biopha.2019.109029

46.  Mediators of Inflammation(2019).miR-23b negatively regulates sepsis-induced inflammatory responses by targeting ADAM10 in human THP-1 monocytes. (IF:3.545)  DOI: 10.1155/2019/5306541

47.  Frontiers in Physiology(2019).Survival motor neuron (SMN) protein insufficiency exacerbates renal ischemia/reperfusion injury. (IF:3.367 ) DOI: 10.3389/fphys.2019.00559

48.  International Immunopharmacology(2019).MiR-150 attenuates LPS-induced acute lung injury via targeting AKT3. (IF:3.361 ) DOI: 10.1016/j.intimp.2019.105794

49.  Eur Rev Med Pharmacol Sci(2019). Long noncoding RNA PCAT6 regulates cell growth and metastasis via Wnt/beta-catenin pathway and is a prognosis marker in cervical cancer. (IF:2.727) DOI: 10.26355/eurrev_201903_17233

50.  BioMed Research International(2019).PC-3-Derived ExosomesInhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-???B Signaling Pathway.  (IF:2.366) DOI: 10.1155/2019/8650846

51.  Cancer Management and Research(2019).Long noncoding RNA FEZF1-AS1 promotes the motility of esophageal squamous cell carcinoma through Wnt/β-catenin pathway. (IF:2.243) DOI: 10.2147/CMAR.S196004

52.  Molecular Medicine Reports(2019).BAFF is involvedin the pathogenesis of IgA nephropathy by activating the TRAF6/NF_x005fκB signaling pathway in glomerular mesangial cells. (IF:2.085)  DOI: 10.3892/mmr.2019.10870

53.  Molecular Biology Reports(2019). Aberrant e­xpressionof long noncoding RNAs in the serum and myocardium of spontaneous hypertensive rats. (IF:1.466 ) DOI: 10.1007/s11033-019-05086-x

54.  Journal of Experimental & Clinical Cancer Research(2018).INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia. (IF:5.189 ) DOI: 10.1186/s13046-018-0675-9

55.  Journal of Endocrinology(2018). SRT2104attenuates diabetes-induced aortic endothelial dysfunction via inhibition of P53. (IF:4.706 ) DOI: 10.1530/JOE-17-0672

56. scientific reports(2018). Sodium selenite inhibitsdeoxynivalenol-induced injury in GPX1-knockdown porcine splenic lymphocytes in culture. (IF:4.149)DOI: 10.1038/s41598-018-36149-x

57.  Biochimica et Biophysica Acta (BBA) - Molecular Cell Research(2018).MDM2 controls NRF2 antioxidant activity in prevention of diabetic kidney disease. (IF:3.982)  DOI: 10.1016/j.bbamcr.2018.04.011

58.  Oncotarget(2018).FC-99 ameliorates sepsis-induced liver dysfunction by modulating monocyte/macrophage differentiation via Let-7a related monocytes apoptosis. (IF:3.765)    DOI: 10.18632/oncotarget.24127

59.  Oncotarget(2018).Metformin synergistically suppress tumor growth with doxorubicin and reverse drug resistance by inhibiting the e­xpression and function of P-glycoprotein in MCF7/ADR cells and xenograft models. (IF:3.765) DOI: 10.18632/oncotarget.23187

60.  Biochemistry and Cell Biology(2018).MMS19 localizes to mitochondria and protects the mitochondrial genome from oxidative damage. (IF:2.25 ) DOI: 10.1139/bcb-2017-0149

61.  Biochimica et Biophysica Acta(2018).A novel brown adipocyte-enrichedlong non-coding RNA that is required for brown adipocyte differentiation and sufficient to drive thermogenic gene program in white adipocytes. (IF:2.014 ) DOI: 10.1016/j.bbalip.2018.01.008

62.  Cell Research(2017).5-methylcytosine promotes mRNA export — NSUN2 as the  methyltransferase and ALYREF as an m5C reader. (IF:13.9 ) DOI: 10.1038/cr.2017.55

63.  Free Radical Biology & Medicine(2017).Epigallocatechin gallateupregulates NRF2 to prevent diabetic nephropathy via disabling KEAP1. (IF:6.078 ) DOI: 10.1016/j.freeradbiomed.2017.04.365

64.  Journal of Molecular Endocrinology(2017).SP600125 suppresses Keap1 e­xpression and results in NRF2-mediated prevention of diabetic nephropathy. (IF:3.577) DOI: 10.1530/JME-17-0260

65.  Oncotarget(2016).Knockdown of Golgi phosphoprotein 2 inhibits hepatocellular carcinoma cell proliferation and motility. (IF:3.765 ) DOI: 10.18632/oncotarget.7271

66.  Oncotarget(2016).Migration-inducing gene7 promotes tumorigenesis and angiogenesis and independently predicts poor prognosis of epithelial ovarian cance. (IF:3.765) DOI: 10.18632/oncotarget.8487

67.  Molecular Nutrition Food Research(2014).Low‐dose diet supplement of a natural flavonoid, luteolin, ameliorates diet‐induced obesity and insulin resistance in mice. (IF:5.426)  DOI: 10.1002/mnfr.201300830

68.  scientific reports(2013). APG: an active protein-gene network model to quantify regulatory signals in complex biological systems. (IF:4.149) DOI: 10.1038/srep01097