Mouse KLF4 Antibody  ，AF3158，R&D：

Mouse KLF4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse KLF4 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant human (rh) KLF4 is observed, and less than 2% cross-reactivity with rhKLF2 and rhKLF5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse KLF4
Ala11-Phe483
Accession # Q60793

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 μg/mL

Recombinant Mouse KLF4

Chromatin Immunoprecipitation (ChIP)

5 μg/5 x 10⁶ cells

See below

Immunocytochemistry

5-15 μg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Chromatin Immunoprecipitation (ChIP) View Larger

Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The B2R promoter was detected by standard PCR.

Immunocytochemistry View Larger

KLF4 in D3 Mouse Cell Line. KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.