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Human PDGF Antibody

Human PDGF Antibody

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商品描述

Human PDGF Antibody，AB-20-NA，R&D:

Human PDGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) PDGF?AA, 60% cross-reactivity with rhPDGF-AB, and nearly 100% cross-reactivity with rhPDGF-BB is observed.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
Human platelet-derived PDGF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications


Recommended Concentration
Sample
Western Blot
1 μg/mL
Human PDGF (Catalog # 120-HD)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize PDGF-induced proliferation in the NR6R?3T3 mouse fibroblast cell line [Rizzino, A. et al. (1988) Cancer Res. 48:4266]. The Neutralization Dose (ND50) is typically 5-10 μg/mL in the presence of 10 ng/mL Human PDGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by PDGF and Neutralization by Human PDGF Antibody. View Larger

Cell Proliferation Induced by PDGF and Neutralization by Human PDGF Antibody. Human PDGF (Catalog # 120?HD) stimulates proliferation in the NR6R?3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Human PDGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human PDGF Polyclonal Antibody (Catalog # AB-20-NA). The ND50 is typically 5-10 μg/mL.


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