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Recombinant Human PDGF-BB Protein, CF
Recombinant Human PDGF-BB Protein, CF

Recombinant Human PDGF-BB Protein, CF

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商品描述
Recombinant Human PDGF-BB Protein, CF，220-BB-010，R&D:

Recombinant Human PDGF-BB Protein, CF Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R?3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.
The specific activity of Recombinant Human PDGF-BB is approximately 5.8 x 103 IU/μg, which is calibrated against recombinant human PDGF-BB WHO International Standard (NIBSC code: 94/728).
Source
E. coli-derived human PDGF-BB protein
Ser82-Thr190
Accession #
Q6FHE7
N-terminal Sequence
Analysis
Ser82
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.3 kDa (monomer)
SDS-PAGE
12 kDa, reducing conditions


Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

220-BB

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R?3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.

SDS-PAGE SDS-PAGE View Larger

1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.